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15.4: Constructing the Biological Profile

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    Who Is It?

    “Who is it?” is one of the first questions that law enforcement officers ask when they are faced with a set of skeletal remains. Likewise, when human bones are found as part of archaeological fieldwork, the remains present the opportunity to learn more about the individuals who lived in the past. In order to answer this question, “who is it?”, bioarchaeologists and forensic anthropologists construct a biological profile (White and Folkens 2005, 405). A biological profile is an individual’s identifying characteristics, or biological information, which include the following: sex, age, stature, ancestry, skeletal variation, trauma, and pathology.

    Although the biological profile is constructed by forensic anthropologists and bioarchaeologists using the same methodology, the estimations of these skeletal characteristics, or attributes, form the framework for different kinds of questions. Forensic anthropologists typically construct a biological profile to help positively identify a deceased person. In some cases, bioarchaeologists match information about a known individual in the past to remains found in an archaeological context, but they generally construct a biological profile to learn more about people’s everyday lives. For example, bioarchaeologists may focus on indicators of pathological conditions in bone to assess the level of stress or disease that affected a particular individual or population. Likewise, bioarchaeologists might assess ancestry to understand more about migration patterns, population history, or relatedness among groups. For an additional example, see the search for, excavation, and analysis of King Richard III’s remains in the box below.


    For an interesting overview of the search for the grave of the English King Richard III, as well as the discovery of the skeletal remains and the subsequent osteological analysis to help identify the remains as King Richard III’s, see The Discovery of Richard III at:

    The following section will lay out each component of the biological profile and briefly review standard methodology used for each.

    Estimating Sex

    Estimation of sex is often one of the first things considered when establishing a biological profile because several other parts, such as age and stature estimations, rely on an estimation of sex to make the calculations more accurate.

    Estimations of sex look at differences in both morphological (form or structure) and metric (measured) traits in individuals. When assessing morphological traits, the skull and the pelvis are the most commonly used areas of the skeleton for estimations. These differences are related to sexual dimorphism usually varying in the amount of robusticity seen between males and females. Robusticity deals with strength and size; it is frequently used as a term to describe a large size or thickness. In general, males will show a greater degree of robusticity than females. For example, the length and width of the mastoid process, a bony projection located behind the opening for the ear, is typically larger in males. The mastoid process is an attachment point for muscles of the neck, and this bony projection tends to be wider and longer in males. In general, cranial features tend to be more robust in males (Figure 15.10).


    Figure \(\PageIndex{1}\): Anterior and lateral view of a male and female cranium.

    When considering the pelvis, the features associated with the ability to give birth help distinguish females from males. During puberty, estrogen causes a widening of the female pelvis to allow for the passage of a baby. Several studies have identified specific features or bony landmarks associated with the widening of the hips, and this section will discuss one such method. The Phenice Method (Phenice 1969) is traditionally the most common reference used to assess morphological characteristics associated with sex. The Phenice Method specifically looks at the presence or absence of (1) a ventral arc, (2) the presence or absence of a sub-pubic concavity, and (3) the width of the medial aspect of the ischiopubic ramus (Figure 15.11). When present, the ventral arc, a ridge of bone located on the ventral surface of the pubic bone, is indicative of female remains. Likewise the presence of a sub-pubic concavity and a narrow medial aspect of the ischiopubic ramus is associated with a female sex estimation. Assessments of these features, as well as those of the skull (when both the pelvis and skull are present), are combined for an overall estimation of sex.

    image28-4.pngFigure \(\PageIndex{2}\): Features associated with the Phenice Method.

    Metric analyses are also used in the estimation of sex. Measurements taken from every region of the body can contribute to estimating sex through statistical approaches that assign a predictive value of sex. These approaches can include multiple measurements from several skeletal elements in what is called multivariate (multiple variables) statistics. Other approaches consider a single measurement, such as the diameter of the head of the femur, of a specific element in a univariate (single variable) analysis (Berg 2017, 152–156).

    It is important to note that, although forensic anthropologists and bioarchaeologists usually begin assessment of biological profile with sex, there is one major instance in which this is not appropriate. The case of two individuals found near Willits, California, on July 8, 1979, is one example that demonstrates the effect age has on the estimation of sex. The identities of the two individuals found in Willits were unknown; therefore, law enforcement sent them to a lab for identification. A skeletal analysis determined that the remains represented one adolescent male and one adolescent female, both younger than 18 years of age. This information did not match with any known missing children at the time.

    In 2015, the cold case was reanalyzed, and DNA samples were extracted. The results indicated that the remains were actually those of two girls who went missing in 1978. The girls were 15 years old and 14 years old at the time of death. It is clear that the 1979 results were incorrect, but this mistake also provides the opportunity to discuss the limitations of assessing sex from a subadult skeleton.

    Assessing sex from the human skeleton is based on biological and genetic traits associated with females and males. These traits are linked to differences in sexual dimorphism and reproductive characteristics between females and males. The link to reproductive characteristics means that most indicators of biological sex do not fully manifest in prepubescent individuals, making estimations of sex unreliable in younger individuals (SWGANTH 2010b). This was the case in the example of the 14-year-old girl. When examined in 1979, her remains were misidentified as male because she had not yet fully developed female pelvic traits.

    Sex vs. Gender

    Biological sex is a different concept than gender. While biological anthropologists can estimate sex from the skeleton, estimating an individual’s gender would require a greater context as gender is culturally defined rather than biologically defined. Take for example an individual who identifies as transgender. This is an individual who has a gender identity that is different from their biological sex. The gender identity of any individual depends on factors related to self-identification, situation, or context, and cultural factors. While in the U.S. we have historically thought of sex and gender as binary concepts (male or female), many cultures throughout the world recognize several possible gender identities. In this sense, gender is seen as a continuous or fluid variable rather than a fixed one.

    Estimating Ancestry

    Ancestry is another component of the biological profile. As noted previously, ancestry can aid law enforcement in their identification of missing persons and can help bioarchaeologists understand many different things about individuals and populations living in the past, such as migration patterns and population distance. Biological ancestry today is often incorrectly labeled as race and generally refers to the individual’s phenotype (outward appearance). Within the field of anthropology, ancestry estimation has a contentious history, and early attempts at racial classification were largely based on the erroneous assumption that an individual’s phenotype was correlated with their innate intelligence and abilities (see Chapter 13 for a more in-depth discussion of the history of the race concept). Biological ancestry refers to the underlying genetic differences between modern populations. In any other organism/living thing, groups divided according to the biological race concept would be defined as a separate species. The major issue with applying the biological race concept to humans is that there are not enough differences between any two populations to separate on a genetic basis. In other words, biological races do not exist in human populations. However, the concept of race has been perpetuated and upheld by sociocultural constructs of race (see Chapter 13).

    The conundrum for forensic anthropologists is the fact that while races do not exist on a biological level, we still recognize and categorize others based on their phenotype. Clearly, our phenotype is an important factor in not only how we are viewed by others but also how we identify ourselves. Also, when a person is reported missing, the information that is collected by law enforcement and sometimes entered into a missing person’s database includes age, biological sex, stature, and “race.” Therefore, the more information a forensic anthropologist can provide regarding the individual’s physical characteristics, the more he or she can help to narrow the search. As an exercise, create a list of all of the women you know who are between the ages of 18 and 24 and approximately 5'4" to 5'9" tall. You probably have several dozen people on the list. Now, consider how many females you know who are between the ages of 18 and 24, approximately 5'4" to 5'9" tall, and are Vietnamese. Your list is going to be significantly shorter. That’s how missing persons searches go as well. The more information you can provide regarding a decedent’s phenotype, the fewer possible matches law enforcement are left to investigate. This is how ancestry has become an indispensable part of the biological profile.

    In an effort to combat the erroneous assumptions tied to the race concept, forensic anthropologists have attempted to reframe this component of the biological profile. The term race is no longer used in casework and teaching. Instead, employing the word ancestry is a more appropriate way to describe an individual’s phenotype, because we are largely shaped by the environments surrounding our recent ancestral origins. In other words, our phenotype lends clues to the environment for which our ancestors were best adapted (for additional information, see Chapters 13 and 14).

    Because human populations vary in their phenotype due to environmental forces, forensic anthropologists are able to use morphological traits to predict the ancestral origins of an unidentified individual. In general, anthropologists are able to divide humans into broad geographically discrete groups, including (but not limited to) the following: European, African, Asian, Native American, and Hispanic. Traditionally, ancestry assessment was accomplished through a visual inspection of morphological variants of the skull (morphoscopics), primarily focused on elements of the facial skeleton, including the nose, eyes, and cheek bones (Figure 15.12). However, in an effort to reduce subjectivity, nonmetric cranial traits are now assessed within a statistical framework to help anthropologists better interpret their distribution among living populations (Hefner and Linde 2018). Based on the observable traits, a macromorphoscopic analysis will allow the practitioner to create a statistically validated prediction of geographic origin. In essence, forensic anthropologists are using human variation in the estimation of geographic origin, by referencing documented frequencies of nonmetric skeletal indicators, or macromorphoscopic traits.

    image18-3.jpgFigure \(\PageIndex{3}\): Skeletal traits commonly used in a morphological assessment of ancestry.

    Finally, ancestry can also be determined through metric analyses. The computer program Fordisc is an anthropological tool used to estimate different components of the biological profile, including ancestry, sex, and stature. When using Fordisc, skeletal measurements are input into the computer software and the program employs multivariate statistical classification methods, including discriminant function analysis, to generate a statistically validated prediction for the geographic origin of unknown remains. Fordisc will also tell the analyst the likelihood of the prediction being correct, as well as how typical the metric data is for the assigned group.

    Estimating Age

    Estimating age from the skeleton relies on the measurement of two basic physiological processes: (1) growth and development and (2) degeneration (or aging). From fetal development on, our bones and teeth grow and change at a predictable rate. This provides for relatively accurate age estimates. After our bones and teeth cease to grow and develop, the bone begins to undergo structural changes, or degeneration, associated with aging. This does not happen at such predictable rates and, therefore, results in less accurate or larger age-range estimations.

    During growth and development stages, two primary methods used for estimations of age of subadults (those under the age of 18) are epiphyseal union and dental development. Epiphyseal union, or epiphyseal fusion, refers to the appearance and closure of the epiphyseal plates between the primary centers of growth in a bone and the subsequent centers of growth (refer to Figure 15.7). Prior to complete union, the cartilaginous area between the primary and secondary centers of growth is also referred as the growth plates (Schaefer et al. 2009). Different areas of the skeleton have documented differences in the appearance and closure of epiphyses, making this a reliable method for aging subadult remains (SWGANTH 2013).

    As an example of its utility in the identification process, epiphyseal development was used to identify two subadult victims of a fatal fire in Flint, Michigan, in February 2010. The remains represented two young girls, ages three and four. Due to the intensity of the fire, the subadult victims were differentiated from each other through the appearance of the patella, the kneecap. The patella is a bone that develops within the tendon of the quadriceps muscle at the knee joint. The patella begins to form around three to four years of age (Cunningham et al. 2016, 407–409). In the example above, radiographs of the knees showed the presence of a patella in the four-year-old girl and the absence of a clearly discernible patella in the three-year-old.

    image25-6.pngFigure \(\PageIndex{4}\): Dental development in a subadult.

    Dental development begins during fetal stages of growth and continues until the complete formation and eruption of the adult third molars (if present). The first set of teeth to appear are called deciduous or baby teeth. Individuals develop a total of 20 deciduous teeth, including incisors, canines, and molars. These are generally replaced by adult dentition as an individual grows (Figure 15.13). A total of 32 teeth are represented in the adult dental arcade, including incisors, canines, premolars, and molars. When dental development is used for age estimations, researchers use both tooth-formation patterns and eruption schedules as determining evidence. For example, the crown of the tooth forms first followed by the formation of the tooth root. During development, an individual can exhibit a partially formed crown or a complete crown but a partially formed root. The teeth generally begin the eruption process once the crown of the tooth is complete. The developmental stages of dentition are one of the most reliable and consistent aging methods for subadults (Langley et al. 2017, 176–177).

    Degenerative changes in the skeleton typically begin after 18 years of age, with more prominent changes developing after an individual reaches middle adulthood (commonly defined as after 35 years of age in osteology). These changes are most easily seen around joint surfaces of the pelvis, the cranial vault, and the ribs. In this chapter, we focus on the pubic symphysis surfaces of the pelvis and the sternal ends of the ribs, which show metamorphic changes from young adulthood to older adulthood. The pubic symphysis is a joint that unites the left and right halves of the pelvis. The surface of the pubic symphysis changes during adulthood, beginning as a surface with pronounced ridges (called billowing) and flattening with a more distinct rim to the pubic symphysis as an individual ages. As with all metamorphic age changes, older adults tend to develop lipping around the joint surfaces as well as a breakdown of the joint surfaces. The most commonly used method for aging adult skeletons from the pubic symphysis is the Suchey-Brooks method (Brooks and Suchey 1990; Katz and Suchey 1986). This method divides the changes seen with the pubic symphysis into six phases based on macroscopic age-related changes to the surface. Figure 15.14 provides a visual of the degenerative changes that typically occur on the pubic symphysis.

    image1-4.pngFigure \(\PageIndex{5}\): Examples of degenerative changes to the pubic symphysis: (A) young adult; (B) middle adult; (C) old adult.

    The sternal end of the ribs, the anterior end of the rib that connects via cartilage to the sternum, is also used in age estimations of adults. This method, first developed by M. Y. İşcan and colleagues, looks at both the change in shape of the sternal end but also the quality of the bone (İşcan et al. 1984; İşcan et al. 1985). The sternal end first develops a billowing appearance in young adulthood. The bone typically develops a wider and deeper cupped end as an individual ages. Older adults tend to exhibit bony extensions of the sternal end rim as attaching cartilage ossifies. Figure 15.15 provides a visual of the degenerative changes that typically occur in sternal rib ends.

    image2-4.pngFigure \(\PageIndex{6}\): Examples of degenerative changes to the sternal rib end: (A) young adult; (B) middle adult; (C) old adult.

    Estimating Stature

    Stature, or height, is one of the most prominently recorded components of the biological profile. Our height is recorded from infancy through adulthood. Doctor’s appointments, driver’s license applications, and sports rosters all typically involve a measure of stature for an individual. As such, it is also a component of the biological profile nearly every individual will have on record. Bioarchaeologists and forensic anthropologists use stature estimation methods to provide a range within which an individual’s biological height would fall. Biological height is a person’s true anatomical height. However, the range created through these estimations is often compared to reported stature, which is typically self-reported and based on an approximation of an individual’s true height (Ousley 1995).

    In June 2015, two men were shot and killed in Granite Bay, California, in a double homicide. Investigators were able to locate surveillance camera footage from a gas station where the two victims were spotted in a car with another individual believed to be the perpetrator in the case. The suspect, sitting behind the victims in the car, hung his right arm out of the window as the car drove away. The search for the perpetrator was eventually narrowed down to two suspects. One suspect was 5'8" while the other suspect was 6'4", representing almost a foot difference in height between the two. Forensic anthropologists were given the dimensions of the car (for proportionality of the arm) and were asked to calculate the stature of the suspect in the car from measurements of the suspect’s forearm hanging from the window. Approximate lengths of the bones of the forearm were established from the video footage and used to create a predicted stature range. Stature estimations from skeletal remains typically look at the correlation between the measurements of any individual bone and the overall measurement of body height. In the case above, the length of the right forearm pointed to the taller of the two suspects who was subsequently arrested for the homicide.

    Certain bones, such as the long bones of the leg, contribute more to our overall height than others and can be used with mathematical equations known as regression equations. Regression methods examine the relationship between variables such as height and bone length and use the correlation between the variables to create a prediction interval (or range) for estimated stature. This method for calculating stature is the most commonly used method (SWGANTH 2012). Figure 15.16 shows the measurement of the bicondylar length of the femur for stature estimations.

    image6-8.pngFigure \(\PageIndex{6}\): Image of measurement of the bicondylar length of the femur, often used in the estimation of living stature.

    Identification Using Individualizing Characteristics

    One of the most frequently requested analyses within the forensic anthropology laboratory is assistance with the identification of unidentified remains. While all components of a biological profile, as discussed above, can assist law enforcement officers and medical examiners to narrow down the list of potential identifications, a biological profile will not lead to a positive identification. The term positive identification refers to a scientifically validated method of identifying previously unidentified remains. Presumptive identifications, however, are not scientifically validated; rather, they are based on circumstances or scene context. For example, if a decedent is found in a locked home with no evidence of forced entry but the body is no longer visually identifiable, it may be presumed that the remains belong to the homeowner. Hence, a presumptive identification.

    The medicolegal system ultimately requires that a positive identification be made in such circumstances, and a presumptive identification is often a good way to narrow down the pool of possibilities. Biological profile information also assists with making a presumptive identification based on an individual’s phenotype in life (e.g., what they looked like). As an example, a forensic anthropologist may establish the following components of a biological profile: white male, between the ages of 35 and 50, approximately 5'7" to 5'11". While this seems like a rather specific description of an individual, you can imagine that this description fits dozens, if not hundreds, of people in an urban area. Therefore, law enforcement can use the biological profile information to narrow their pool of possible identifications to include only white males who fit the age and height outlined above. Once a possible match is found, the decedent can be identified using a method of positive identification.

    Positive identifications are based on what we refer to as individualizing traits or characteristics, which are traits that are unique at the individual level. For example, brown hair is not an individualizing trait as brown is the most common hair color in the U.S. But, a specific pattern of dental restorations or surgical implants can be individualizing, because it is unlikely that you will have an exact match on either of these traits when comparing two individuals.

    A number of positive methods are available to forensic anthropologists, and for the remainder of this section we will discuss the following methods: comparative medical and dental radiography and identification of surgical implants.

    image12-7.pngFigure \(\PageIndex{7}\): Example of the unique shape of the frontal sinus.

    Comparative medical and dental radiography is used to find consistency of traits when comparing antemortem records (medical and dental records taken during life) with images taken postmortem (after death). Comparative medical radiography focuses primarily on features associated with the skeletal system, including trabecular pattern (internal structure of bone that is honeycomb in appearance), bone shape or cortical density (compact outer layer of bone), and evidence of past trauma or skeletal pathology. Other individualizing traits include the shape of various bones or their features, such as the frontal sinuses (Figure 15.17).

    Comparative dental radiography focuses on the number, shape, location, and orientation of dentition and dental restorations in antemortem and postmortem images. While there is not a minimum number of matching traits that need to be identified for an identification to be made, the antemortem and postmortem records should have enough skeletal or dental consistencies to conclude that the records did in fact come from the same individual (SWGANTH 2010a). Consideration should also be given to population-level frequencies of specific skeletal and dental traits. If a trait is particularly common within a given population, it may not be a good trait to utilize for positive identification.

    image8-8.pngFigure \(\PageIndex{8}\): Image of joint replacement in the right shoulder.

    Surgical implants or devices can also be used for identification purposes (Figure 15.18). These implements are sometimes recovered with human remains. One of the ways forensic anthropologists can use surgical implants to assist in decedent identification is by providing a thorough analysis of the implant and noting any identifying information such as serial numbers, manufacturer symbols, and so forth. This information can then sometimes be tracked directly to the manufacturer or the place of surgical intervention, which may be used to identify unknown remains (SWGANTH 2010a).

    This page titled 15.4: Constructing the Biological Profile is shared under a CC BY-NC 4.0 license and was authored, remixed, and/or curated by Beth Shook, Katie Nelson, Kelsie Aguilera, & Lara Braff, Eds. (Society for Anthropology in Community Colleges) via source content that was edited to the style and standards of the LibreTexts platform.